Microbiological testing for unknown (salmonella sp.)

To begin my unknown research, I prepared and inoculated, with unknown #24, 2 NA slants, 1 NA plate and boiled and cooled 1 slant of FTM. I then placed the FTM, plate (Quadrant streaked) and one of the slants into the incubator, and the other slant into the hood. The slants were inoculated using the glide inoculation technique. After 24 hrs I observed beaded, smooth and raised growth in both slants. On the quad streaked plate I observed round colonies …

…from my lab manual that the unknown #24 was a citrobacter. At that time, I referenced the Bergey's Manual of Determinative Bacteriology, which assisted in my correctly identifying my unknown as Salmonella. I was able to determine that the Citrobacter does not have a capsule as Salmonella does. Finally, I inoculated an Enterotube with unknown #24. After 12 hrs,. I was able to verify with the results of the Enterotube, that I had chosen Salmonella as my unknown.