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Bacterial Conjugation Experiment
BACTERIAL CONJUGATION EXPERIMENT
INTRODUCTION: Bacteria, in general, reproduce asexually, but in order to increase diversity, they have developed a mechanism for transfer of genetic material from one bacterium to another. The ability to perform this transfer is conferred by a set of genes which are called F for 'fertility.' These genes exist on a small, circular piece of deoxyribonucleic acid (DNA) that replicates independently from the bacterial chromosome, or they can be integrated into …
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…minutes 14 NG TNTC NG
Histadine deficient plates
Time 10-1 10-2 10-3 10-4
0 minutes 10 2 NG NG
10 minutes 46 43 26 3
20 minutes TNTC 136 NG 4
30 minutes TNTC 184 8 3
40 minutes TNTC 340 20 NG
50 minutes 45 TNTC 27 3
60 minutes 41 250 42 NG
TNTC = Too numerous to count
NG = No growth
Figure 4 - Time of entry table (percentages of recombinants)
Time Thr - Pro - His -
0 minutes .44 0 0
10 minutes 5.8 2 .02
20 minutes 6.7 2.6 .06
30 minutes .004 1.8 .08
40 minutes .0098 4.3 .09
50 minutes 1.9 2.3 .002
60 minutes 2.4 0 .11
Figure 5 - Time of entry graph (percentages of recombinants)
Figure 6 - Map of E. coli K12
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